Thesis topic proposal
Csaba Tömböly
Semisynthesis of fluorescent protein derivatives for studying membrane proteins


Institute: University of Szeged
theoretical medicine
Theoretical Medicine Doctoral School

Thesis supervisor: Csaba Tömböly
Location of studies (in Hungarian): Laboratory of Chemical Biology, Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences
Abbreviation of location of studies: BRC

Description of the research topic:

GPI-anchored proteins are special membrane associated proteins containing a complex glycolipid that anchors the attached protein to the outer leaflet of the cell membrane. The responsibility of the candidate includes the preparation of protein conjugates containing lipid moieties with small molecule fluorophores, fluorescent microscopy of the resulting fluorescent lipidated proteins, and the investigation of protein-protein interactions of the semisynthetic proteins. After optimizing the protein – lipid conjugation, NMR structural studies will be conducted on membrane anchored N-15 labelled proteins. This way the structural features of proteins in their native environment will be described. Further task of the candidate is the investigation of the structural and kinetic properties of the cyclodextrin inclusion complexes of cholesteryl lipoproteins.

Required language skills: english
Number of students who can be accepted: 1

Deadline for application: 2019-12-31

All rights reserved © 2007, Hungarian Doctoral Council. Doctoral Council registration number at commissioner for data protection: 02003/0001. Program version: 2.2358 ( 2017. X. 31. )