Thesis supervisor: András Dinnyés
co-supervisor: Julianna Kobolák
Location of studies (in Hungarian): Szent István University Abbreviation of location of studies: SZIU
Description of the research topic:
Replacement of animal models in toxicology is an economically and ethically sound target, which can be achieved by using in silico and in vitro approaches. Animal and human cell reporter cell lines need to be developed and validated to reach that overall goal. The project will involve the incorporation of reporter constructs into selected iPSC lines using CRISPR/Cas technology to generate fluorescent reporters of toxicity pathways, which can be then differentiated into specific organotypic cell lineages. Genes within the Nrf2 and/or p53 pathways will be initially targeted. Depending on the progress, multiple labels may be attempted and other pathways investigated. Then the response of undifferentiated iPSC to test compounds will be evaluated in order to compare the responses of the reporter and its parent lineage. The work will therefore involve biomolecular approaches, cell culture and the use of viability assays, stress assays, transcriptomics and kinetic assays.
The scope of the current PhD studentship would be the investigation of the potential of reporter iPSC lines in the development of efficient cellular toxicology models.
Required language skills: English Number of students who can be accepted: 1
Deadline for application: 2017-05-31
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
Login
