Thesis supervisor: István Krizbai
Location of studies (in Hungarian): MTA SZBK Biofizikai Intézet Abbreviation of location of studies: SZBK
Description of the research topic:
By forming a single cell layer lining the blood vessels of the brain, cerebral endothelial cells
(CECs) constitute the principal component of the blood-brain barrier (BBB). Tight junctions (TJ) and adherens junctions (AJ) play a key role in the maintenance of the barrier function. Despite considerable experimental efforts the molecular organization and regulation of cerebral interendothelial junctions is less well understood. Our project is focused on the role of interendothelial junctions under pathological conditions. The experiments will be carried out on an in vitro model of the BBB based on the culture of cerebral endothelial cells. Using different molecular, biochemical and immunofluorescent techniques we will investigate the changes in the expression, localization, interaction and posttranslational modifications of the junctional proteins (occludin, claudins, ZO-1, ZO-2, cadherins, catenins) in CECs in response to pathological conditions like oxidative stress, inflammatory stimuli or cellular transmigration. Proteomic analysis will be applied to find endothelial proteins involved in these pathological processes. Furthermore, planned experiments are designed to elucidate the role of different signaling pathways, protein phosphorylation and proteolysis in the pathomechanism of endothelial dysfunction.
Number of students who can be accepted: 1
Deadline for application: 2017-07-31
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
Login
