témavezető: Talián Csaba Gábor
helyszín (magyar oldal): PTE ÁOK Pécs, Szigeti u. 12. helyszín rövidítés: ÁOK
A kutatási téma leírása:
The members of the actin-binding tropomyosin family display a high structural similarity. While their expression is strictly regulated in space and developmental state, several isoforms are always present in the same cell type, and little is known about their division of labour. Tropomyosins can influence the stability and dynamics of actin filaments; however, their actual biological significance may be the modification of association and function of other actin-binding proteins. The aim of the present research in our institute is to in vitro express tropomyosin isoforms in order to reveal their interactions with other actin-binding partners, like gelsolin, cofilin, twinfilin, caldesmon, myosins etc. The measurements will be carried out after fluorescent labelling by spectroscopy methods and light microscopy. We also intend to express fluorescent proteins even in living neurons. The Ph.D. student will have the opportunity to acquire substantial expertise in various methods from protein cloning through molecular biology techniques to the above mentioned measurement procedures.
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