Highly integrated genome level examination of transcription factor cascades during alternative macrophage polarization using next-generation sequencing method
témavezető: Nagy László
helyszín (magyar oldal): University of Debrecen helyszín rövidítés: DEÁOK
A kutatási téma leírása:
Our research group has been examining the gene regulation of different macrophages by applying next-generation sequencing (NGS)methods such as ATAC-seq, ChIP-seq, GRO-seq and RNA-seq. ChIP-seq (and indirectly also ATAC-seq) allow(s) the whole genomelevel observation of the DNA-binding of chromatin proteins e.g. transcription factors (TFs); and with the use of NGS methods examining gene transcription (GRO-seq and RNA-seq) it can also be determined which genes are regulated by the individual binding sites. Previously we have shown how RXR contributes to the gene regulation of mouse bone marrow-derived macrophages. In this project we would like to examine the development of IL-4 treated, alternatively polarized macrophages and the role of RXR in this process. Our goal is to find out that following STAT6, which TFs contribute to this differentiation. It is known that EGR2 participates in alternative polarization, and according to our newest results the PPARg:RXR heterodimer also plays a role in this process. Beside the mouse-derived data we have NGS data from IL-4-treated human monocyte-derived macrophages too. The combined use of human and mouse data provides a unique opportunity for the interspecies comparison of gene regulation to determine evolutionary conservation. Beside the in vitro models we are proposing to extend our studies to in vivo skeletal muscle repair macrophages. We think that the understanding of the interactions and dynamics of TFs and their cistromes regulating alternative polarization and muscle healing might provide general regularities for the
researchers of the rapidly developing functional genomics.
Jelentkezési határidő: 2019-01-31
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
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