Goda Katalin
The coupling between substrate binding and ATP hydrolysis in P-glycoprotein and ABCG2


Intézmény: Debreceni Egyetem
elméleti orvostudományok
Molekuláris sejt- és immunbiológia Doktori Iskola

témavezető: Goda Katalin
helyszín (magyar oldal): University of Debrecen, Faculty of Medicine
helyszín rövidítés: DEÁOK

A kutatási téma leírása:

One of the unresolved problems of cancer as well as of AIDS chemotherapy is the resistance of target tissues against a broad range of compounds applied in the treatment protocols, such as steroids, antineoplastic drugs, immuno-suppressive agents or HIV protease inhibitors. This phenomenon is frequently associated with and caused by the overexpression of certain ABC (ATP Binding Cassette) transporters, including P-glycoprotein (Pgp, MDR1, ABCB1) and breast cancer resistance protein (ABCG2; BCRP), that can actively efflux the chemotherapeutic drugs from the cells leading to a decreased accumulation of drugs in these tissues.
Despite the large body of structural and functional data regarding the ABC transporter proteins, it is still unknown how ATP binding and hydrolysis is coordinated between the two nucleotide binding domains and how drug transport process is coupled to nucleotide binding and hydrolysis. Therefore, the major aim of our project proposal is to study various aspects of these processes in Pgp and ABCG2 utilizing our unique repertoire of biophysical and biochemical tools applicable for systematic examination of the conformation and drug binding affinity changes upon distinct steps of the catalytic cycle.

felvehető hallgatók száma: 1

Jelentkezési határidő: 2018-05-15

Minden jog fenntartva © 2007, Országos Doktori Tanács - a doktori adatbázis nyilvántartási száma az adatvédelmi biztosnál: 02003/0001. Program verzió: 2.2358 ( 2017. X. 31. )