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Krizbai István
Molecular basis of the blood-brain barrier function under physiological and pathological conditions

TÉMAKIÍRÁS

Intézmény: Szegedi Tudományegyetem
elméleti orvostudományok
Elméleti Orvostudományok Doktori Iskola

témavezető: Krizbai István
helyszín (magyar oldal): MTA SZBK Biofizikai Intézet
helyszín rövidítés: SZBK


A kutatási téma leírása:

By forming a single cell layer lining the blood vessels of the brain, cerebral endothelial cells
(CECs) constitute the principal component of the blood-brain barrier (BBB). Tight junctions (TJ) and adherens junctions (AJ) play a key role in the maintenance of the barrier function. Despite considerable experimental efforts the molecular organization and regulation of cerebral interendothelial junctions is less well understood. Our project is focused on the role of interendothelial junctions under pathological conditions. The experiments will be carried out on an in vitro model of the BBB based on the culture of cerebral endothelial cells. Using different molecular, biochemical and immunofluorescent techniques we will investigate the changes in the expression, localization, interaction and posttranslational modifications of the junctional proteins (occludin, claudins, ZO-1, ZO-2, cadherins, catenins) in CECs in response to pathological conditions like oxidative stress, inflammatory stimuli or cellular transmigration. Proteomic analysis will be applied to find endothelial proteins involved in these pathological processes. Furthermore, planned experiments are designed to elucidate the role of different signaling pathways, protein phosphorylation and proteolysis in the pathomechanism of endothelial dysfunction.

felvehető hallgatók száma: 1

Jelentkezési határidő: 2017-07-31

 
Minden jog fenntartva © 2007, Országos Doktori Tanács - a doktori adatbázis nyilvántartási száma az adatvédelmi biztosnál: 02003/0001. Program verzió: 1.2357 ( 2017. V. 15. )