Antimicrobial resistance (AMR) poses a significant threat to human health worldwide. Resistance of Gram-negative bacilli to carbapenem and third generation cephalosporin antibiotics is ranked as critical priority for research by the World Health Organisation. Resistance to these antibiotics is often conferred by genes located on mobile genetic elements, e.g., plasmids or transposons. We aim to study human and animal isolates of Gram-negative bacilli with such resistance phenotype to map mobile genetic elements carrying carbapenem and 3rd generation cephalosporin resistance genes. We also would like to assess what other resistance determinants (antibiotic, disinfectant or heavy metal) are co-located on these mobile genetic elements, which may promote their spread.