Identification and genetic characterisation of potential new senescence and cell death related mechanisms during salivary gland disruption in Drosophila
Thesis supervisor: Tamás Csizmadia
Location of studies (in Hungarian): Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Abbreviation of location of studies: ELTE
Description of the research topic:
"During pupariation the larval tissues, such as the salivary gland of Drosophila melanogaster are degraded within 16-18 hours after puparium formation (APF) by the interplay of autophagic and programmed cell death mechanisms. Therefore, this organ is a frequently used model system to study these cell biological processes. Importantly, the ultrastructure of the dying salivary gland remains a mystery, because the dissection of this organ right before its disruption is too difficult. Using the secretory granule/crinosome (secondary lysosome created by the direct fusion of secretory granules and late endosomes/lysosomes specific pH resistant DsRed tagged glue protein (Sgs3-DsRed) expressing animals, we can follow the developmental state of salivary gland and dissect this organ from the pupa at very late time points (15-18 hours APF). We also use toluidine blue for staining light microscopic samples, which show several interesting phenomena - dark patches near the crinosomes, chromatin condensation and formation of blebs containing cytosolic components, crinosomes with fibrous inner content - in the dying salivary glands during the pupal development. Hereinafter, we will also examine these samples by electron microscope and molecular genetic analysis to identify genes, which regulate thes potential new cell death releted mechanisms.
The candidate have to take care of the mutant and transgenic fly stocks, to make crossings, isolate salivary gland samples and perform PCR, Western blot, light and electron microscopical experiments and immunocytochemistry."
Required language skills: English Further requirements: Degree in Genetics, Cell and Developmental Biology (MSc)
Number of students who can be accepted: 1
Deadline for application: 2024-05-31
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
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