Thesis supervisor: György Várady
Location of studies (in Hungarian): HUN-REN Research Centre for Natural Sciences, Institute of Molecular Life Sciences Abbreviation of location of studies: H-REN
Description of the research topic:
"In this project we plan to develop a new, modern, high-throughput flow cytometry methodology to investigate the transport capacity of ABC membrane proteins in human lymphocyte and granulocyte populations obtained from the blood samples of volunteers.
ABC transporters play an important physiological role in the secretion of endogenous substrates, including cytokines and hormones, and may therefore play an important role in chronic inflammatory processes.
As a background to this work, some fluorescent compounds that are commercially available but not known to be used as ABC transporter substrates have been characterized by flow cytometry.
These compounds are distinguished from the dyes used in current functional assays by their very slow ability to cross the membrane of living cells. Conventional substrates passively enter the cytoplasm within minutes, so cells with poor transport function cannot remove them in real time, making these cells appear mostly transporter-negative.
Due to their slow uptake, the dyes we use are able to pump much less transport protein out of the cells, making it a good choice for more sensitive assays.
Using the appropriate CD markers (e.g. CD3, CD4, CD8, CD66) together, the transport function of major lymphocyte and granulocyte populations and their subpopulations, as well as the amount of cell surface ABC proteins can be accurately determined.
The more well-known polymorphisms of ABCB1 and ABCG2 were examined by TaqMan PCR, and the results were compared with the transport function and expression. In this way, the basics of a complex methodology, which will later be suitable for the study of many diseases, will be developed.
Required language skills: English, Hungarian Further requirements: laboratory practice, good problem solving skills
Number of students who can be accepted: 1
Deadline for application: 2024-05-31
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
Login
