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Thesis topic proposal
 
Gábor Kovács L.
Analysis of circulating cell-free DNA and miRNA as pathogenetic markers in liquid biopsy

THESIS TOPIC PROPOSAL

Institute: University of Pécs
clinical medicine
Doctoral School of Clinical Medicine

Thesis supervisor: Gábor Kovács L.
Location of studies (in Hungarian): PTE ÁOK Pécs, Szigeti u. 12.
Abbreviation of location of studies: ÁOK


Description of the research topic:

Circulating cell-free DNA with both nuclear or mitochondrial origin and exosomal gene expression regulatory miRNAs get into the body fluids either by cellular destruction or active secretion. Recognition of these molecular fractions in biological fluids has generated an interest for their potential use as markers for genetic disorders or progressive human diseases. Quantitative and structural analysis of circulating cell-free DNA and exosomal miRNA from body fluid has emerging role as liquid biopsy-based markers. The study focuses on cfDNA and miRNA preparation and subsequent analysis on spent IVF embrional culture media and human liquid biopsy samples. Methods enroll high throughput molecular techniques including fluorescent quantitative real-time PCR, droplet digital PCR and next generation sequencing.

Number of students who can be accepted: 1

Deadline for application: 2023-05-19

 
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