Thesis topic proposal
Miklós Nyitrai
The investigation of protein function and conformation by using biophysical methods


Institute: University of Pécs
theoretical medicine
Doctoral School of Interdisciplinary Medical Sciences

Thesis supervisor: Miklós Nyitrai
Location of studies (in Hungarian): PTE ÁOK Pécs, Szigeti u. 12.
Abbreviation of location of studies: ÁOK

Description of the research topic:

The actin cytoskeleton plays essential roles in many cellular functions. The appropriate time and space control of these processes is critical for most of the cell functions, and manifested by more than 60 families of actin-binding proteins. The scientific questions of the students joining our research group will be centred around the many - yet unknown - details of these regulatory mechanisms. One of the major components of this education will be the understanding of the known mechanisms and analysing the corresponding part of the literature. As part of the process the students are expected to attend national and international conferences and workshops. After defining the research questions we will apply biochemical and molecular biology methods to purify the chosen proteins. The investigations will be carried out by using various biophysical methods, including many assays in fluorescence spectroscopy (both steady-state and time dependent), fluorescence microscopy (conventional, confocal, fluorescence lifetime imaging), calorimetry and rapid kinetic methods. The concept will be to find and describe molecular mechanisms in in vitro experiments, and then correlate them to functions and interactions in living cells. Considering the nature of these research topics the projects are available and suggested for students with background in either medical or natural sciences.

Number of students who can be accepted: 1

Deadline for application: 2023-05-19

All rights reserved © 2007, Hungarian Doctoral Council. Doctoral Council registration number at commissioner for data protection: 02003/0001. Program version: 2.2358 ( 2017. X. 31. )