Thesis supervisor: Ágnes Tantos
Location of studies (in Hungarian): Institute of Enzymology, Research Centre for Natural Sciences Abbreviation of location of studies: ELKH
Description of the research topic:
Results of recent years have shown the widepsread lyine methylation in the human proteome, but its physological relevance and the details of the molecular mechanism remain elusive. In parallel, several histone lysine methyltransferases are known to have non-histone substrates. The goal of the PhD project is the identification of these non-histone substrates of selected proteins. The first subject is KMT2D, but the study of KMT2F, KMT2G and DOT1L is also planned. The tasks of the PhD student involve the generation of siRNA silenced and stable KO cell lines and the identification of lysine-methylated proteins through immunoprecipitation and mass spectrometry, and the verification of the selected targets with targeted experiments.
Required language skills: English Further requirements: Advanced molecular biology studies, experience with maintaining and transfecting mammalian cell lines.
Number of students who can be accepted: 1
Deadline for application: 2023-05-31
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
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