Thesis supervisor: Tamás Arányi
Location of studies (in Hungarian): Semmelweis Egyetem Molekuláris Biológiai Tanszék Abbreviation of location of studies: SE
Description of the research topic:
Ablation of DNA methyltransferases (Dnmt) and oxidative DNA demethylases (Tet1-3) are lethal or lead to developmental defects. Currently, we are investigating the tissue-specific function of Dnmts in the liver. The liver plays an important role in metabolism and detoxification, therefore it is influenced by environmental stress factors and it also effects the function of other organs. The Western-type high-fat high-carbohydrate (HFD) diet leads to obesity, non-alcoholic fatty liver disease (NAFLD), and even hepatocellular carcinoma, which has an ever increasing incidence and is becoming one of the leading death causes among cancers. DNA methylation changes are known to undergo important alterations in these diseases. Therefore, we have decided to investigate the role of the two de novo DNA methyltransferases Dnmt3a and Dnmt3b during liver maturation, under environmental stress conditions and in transgenerational inheritance models in liver-specific knock mice. In our studies we'll use macroscopic, microscopic (histologic, immunhistochemistry), biochemical (Western-blot and blood parameters), molecular biologic (RT-qPCR, bisulfite sequencing, ChIP) methods. Further, we'll also characterize the changes in transcriptome and epigenome and the transgenerational effects.
Required language skills: English Number of students who can be accepted: 2
Deadline for application: 2023-05-31
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
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