Thesis topic proposal
András Szabó
Investigation of the effects of Fumonisin B1 with complex, hybrid imaging techniques (PET-MR) and biochemical methods


Institute: University of Kaposvár
animal husbandry
Doctoral School of Animal Science

Thesis supervisor: András Szabó
Location of studies (in Hungarian): Kaposvár University, Faculty of Agricultural and Environmental Sciences
Abbreviation of location of studies: AKKMÉ

Description of the research topic:

Fumonisin B1 is a characteristic cell membrane modification inducer mycotoxin, occurring mostly on corn and in corn based complete feeds. According to our preliminary results, FB1 induces hepatocellular membrane damage, pulmonary edema and red cell membrane compositional alterations (lipid profile and enzyme activities). In the planned research we intend to describe the effects of FB1 onto the liver and the gastrointestinal tract by means of PET-MR and 2-deoxy-2-(18F)fluoro-D-glucose tracer, to follow up the uptake, the accumulation and the metabolism of labelled glucose in intoxicated model animals. Flow cytometry is planned to be invented to analyse the red cell membrane damage, in particular the Annexin V associated apoptosis, which will be traced as well with gas chromatography. Results expected will aid the better recognition of the harmful effect of FB1 in mammalian species.
Methodological background
lipid analysis (chromatography), enzyme activity measurement with spectrophotometry, flow cytometry, PET-MR

Required language skills: angol
Number of students who can be accepted: 1

Deadline for application: 2021-05-31

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