Thesis supervisor: Csaba Vágvölgyi
co-supervisor: Miklós Takó
Location of studies (in Hungarian): Department of Microbiology, Faculty of Science and Informatics, University of Szeged Abbreviation of location of studies: SZTE
Description of the research topic:
Microbial hydrolases (eg. cellulases, lipases, proteases, chitinases) find promising applications in a wide range of industrial processes; however, development of biotechnological methods appropriate for environmental and food-industrial expectations requires identification of novel enzymes/producing isolates. These studies extend to characterization of producing parameters as well as biochemical properties of the new enzymes. Proper analysis of hydrolases involves high-yield fermentation and purification as well. In the course of this program, we analyze the hydrolase production of microorganisms (especially filamentous fungi) using plant-derived waste materials. Besides identification and characterization of new enzyme producing isolates/enzymes, we study the possibilities and biological requirements of the applicability of isolates, which have been found to have significant enzyme production and fermentation properties. This program also extends to preparatory studies for heterologous expression of microbial hydrolases.
Our relevant publications:
1. Kotogán A, Zambrano C, Kecskeméti A, Varga M, Szekeres A, Papp T, Vágvölgyi C, Takó M: An organic solvent-tolerant lipase with both hydrolytic and synthetic activities from the oleaginous fungus Mortierella echinosphaera. International Journal of Molecular Sciences 19:(4) 1129. (2018)
2. Takó M, Kotogán A, Papp T, Kadaikunnan S, Alharbi NS, Vágvölgyi Cs: Purification and properties of extracellular lipases with transesterification activity and 1,3-regioselectivity from Rhizomucor miehei and Rhizopus oryzae. Journal of Microbiology and Biotechnology 27:(2) pp. 277-288. (2017)
3. Takó M, Kotogán A, Krisch J, Vágvölgyi Cs, Mondal KC, Papp T: Enhanced production of industrial enzymes in Mucoromycotina fungi during solid-state fermentation of agricultural wastes/by-products. Acta Biologica Hungarica 66:(3) pp. 348-360. (2015)
4. Kotogán A, Németh B, Vágvölgyi Cs, Papp T, Takó M: Screening for extracellular lipase enzymes with transesterification capacity in Mucoromycotina strains. Food Technology and Biotechnology 52:(1) pp. 73-82. (2014)
Required language skills: English Further requirements: MSc diploma from relevant fields (e.g. Biology, Food engineering, Biotechnology)
Number of students who can be accepted: 1
Deadline for application: 2020-12-31
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
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