Thesis supervisor: Petra Hartmann
Location of studies (in Hungarian): SZTE ÁOK Sebészeti Műtéttani Intézet Abbreviation of location of studies: ÁOK
Description of the research topic:
Our previous studies provided a comprehensive view on the biological effects of exogenous methane (CH4) in various experimental ischemia-reperfusion (IR) and inflammatory settings. However, the underlying mechanisms are still not known with certainty; and the immediate challenge is therefore to test hypotheses to understand the background of CH4-induced reactions. The main purpose of the next studies is to examine the effects of CH4 on possible cellular and subcellular targets, including mitochondria, and to apply CH4 in complex, in vivo animal models of IR, focusing on specific organ functions.
Techniques available in the lab: in vivo animal models (rodents and large animals, minipigs), in vivo imaging techniques with fluorescent laser-scanning endomicroscopy or intravital microscopy. Microcirculatory measurements with orthogonal polarization spectral imaging and laser-Doppler technique. Ex vivo organ perfusion systems (liver, kidney) for static and dynamic graft preservation. Measurement of mitochondrial respiration, hydrogen-peroxide production and membrane potential using high-resolution respirometer and its fluorescent modules (Oroboros Oxygraph 2k). Basic laboratory and biochemical techniques (spectrophotometry, isolation of mitochondria).
Required language skills: angol B2 komplex nyelvvizsga Recommended language skills (in Hungarian): English B2 level/TOEFL Further requirements: previous scientifc research work
Number of students who can be accepted: 2
Deadline for application: 2020-08-31
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
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