Thesis topic proposal
Endogenous and exogenous cardioprotective mechanisms in metabolic diseases


Institute: University of Szeged
theoretical medicine
Doctoral School of Multidisciplinary Medical Scienses

Thesis supervisor: Gergő Szűcs
Web address (URL): http://biochem.szote.u-szeged.hu
Location of studies (in Hungarian): University of Szeged, Faculty of Medicine, Dept. of Biochemistry
Abbreviation of location of studies: ÁOK

Description of the research topic:

Ischemic heart diseases are the leading cause of death in developed and developing countries. Several risk factors can be responsible for their development, including co-morbidities such as obesity, metabolic syndrome, or type 2 diabetes mellitus. In Hungary, the proportion of overweight people is 30.8%, while the proportion of obese people is 36.5%. The prevalence of metabolic syndrome in the adult population (20–69 years) is 26% in men and 24.1% in women. Diabetes affects 8.5% of the population. In these diseases, dysfunction occurs in the adipose tissue due to nutrient overload and insulin resistance. As a consequence of dysfunction, metabolic diseases alter the levels of adipokines and mitochondrial peptides (MDPs). Adipokines are cytokines produced by adipose tissue, whose primary function is to influence glucose and lipid homeostasis, but also play a role in inflammatory processes. Mitochondrially derived peptides are short peptides encoded in the mitochondrial genome known to affect tissue sensitivity and glucose metabolism. We aim is to investigate the role of adipokines and mitochondrially derived peptides in the induction of endogenous and exogenous cardioprotective mechanisms and to investigate the biochemical background in animal models of metabolic diseases.
Methods: Development of metabolic syndrome and type 2 diabetes animal models and their treatment in experimental animals. Measurement of fasting glucose, oral glucose tolerance and insulin tolerance. Examination of cardiac function with in-vivo PV catheter and in-vitro Neely working heart system. Measurement of infarct size with in-vivo and ex-vivo ischemia-reperfusion models. Histological methods, measurement of metabolites, mRNA and protein levels and enzyme activity with general biochemical and molecular biological methods (colorimetric methods, qRT-PCR, western blot, ELISA)

Required language skills: English B2
Further requirements: 
TDK munka

Number of students who can be accepted: 1

Deadline for application: 2019-12-15

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