Thesis supervisor: Máté Varga
Location of studies (in Hungarian): Eötvös Loránd University, Department of Genetics Abbreviation of location of studies: ELTE
Description of the research topic:
The presence of uracil in DNA is usually the sign of erroneous polymerase activity (dUTP being incorporated instead of dTTP) or cytosine deamination. As the latter results in mutagenic U:G mismatches in the double helix, cells have evolved error repair mechanisms to ensure that such mismatches are repaired promptly, and the freely available dUTP pool is kept at relatively low steady-state levels, to prevent uracil incorporation into the DNA. Recent data suggests that the impairment or absence of the two enzymes that guard the cell from permanent uracil accumulation, uracil–DNA glycosylase (UNG) and dUTPase, can have profound morphological consequences on the development of animals. Using zebrafish (Danio rerio) as a model, in collaboration with Prof. Beáta Vértessy’s group (MTA-TTK), the successful applicant will explore the dynamics of uracil incorporation into the DNA during development, and taking advantage of combined loss-of-function and gain-of-function techniques, (s)he will explore the long-term developmental and genomic consequences of UNG and dUTPase impairments.
Required language skills: fluent English (at least B2) Further requirements: Experience in molecular and developmental genetics (developmental biology, animal models, biochemistry, molecular biology) is an advantage. Interested applicants are advised to send a CV (Europass format) and a motivation letter.
Deadline for application: 2018-05-31
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
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