Thesis supervisor: Tamás Kőszegi
Location of studies (in Hungarian): PTE ÁOK Pécs, Szigeti u. 12. Abbreviation of location of studies: ÁOK
Description of the research topic:
It is well known that dietary carotenoids, flavonoids, polyphenols and many other molecules – primarily antioxidants – exert a significant effect in prevention of chronic degenerative diseases (malignancies, autoimmune processes, cardiovascular diseases, etc). In spite of the large body of evidences obtained from epidemiological studies, from in vitro models and animal experiments the mechanism of cytoprotection is not completely understood. It may be postulated – and experimentally it is already partially verified – that the major cytoprotective factor lies in the antioxidant feature of the studied molecules. On the other hand, the interpretation of the data is often difficult because most authors use only single, purified factors while most probably the favorable effects depend on the synergistic action of a combination of many molecules. The proposed research focuses partly on the already known antioxidants and partly tries to characterize the combined effects of a mixture of protective factors. An in vitro tissue culture model will be established using adherent cell types (e.g. fibroblast, HepG-2, MDCK, etc) and also cells in suspension (e.g. Jurkat, K-562). Cytoprotection will be studied after induction of oxidative stress (e.g. by addition of menadione, NaF, ochratoxin A) by multiparametric luminescent viability testing (ATP, DNA, protein, esterase activity) and fluorescence spectroscopic methods where cells are labeled with oxidant-sensitive dyes. The luminescent techniques will be adapted to microplate reader and flow cytometric analyses. Another part of the experiments is monitoring of the changes in the cell signaling protein pattern of the treated cells using SDS-PAGE and chemiluminescent Western blotting.
Number of students who can be accepted: 1
Deadline for application: 2018-05-18
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
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