Thesis supervisor: Tamás Kőszegi
Location of studies (in Hungarian): PTE ÁOK Szigeti u. 12. Abbreviation of location of studies: ÁOK
Description of the research topic:
Type 2 diabetes mellitus affects hundreds of millions of people worldwide. There is a wide choice of antidiabetic herbs used in complementary treatment on the basis of ethnobotanical observations. The majority of the applied herbs are rich in antioxidants but to our latest knowledge solely the antioxidant feature is not selective and is also not effective in decreasing the symptoms of diabetes mellitus. A potential tool for solving the problem is to reduce insulin resistance at the initial stage of the disease. Intracellular glucose content and/or uptake can easily be measured in tissue culture models by luminescent glucose determination and by uptake of fluorescent glucose analogues using fluorescent microscopy, flow cytometry and spectroscopic techniques. Moreover, it is possible to detect insulin-like effects by following the translocation of GLUT4 transporter with immune fluorescence technique. Anchorage dependent cells (e.g. HepG-2, liver origin) and suspension cultures will be used. Effects of aqueous and apolar extracts of test herbs on the cells will be studied by multiparametric viability-cytotoxicity assays, antioxidant capacity tests and total intracellular glucose/glucose uptake determinations. Plant extracts might affect cell signaling pathways as well, these will be analyzed by immune fluorescence and/or western blotting.
Number of students who can be accepted: 1
Deadline for application: 2018-05-18
2024. IV. 17. ODT ülés Az ODT következő ülésére 2024. június 14-én, pénteken 10.00 órakor kerül sor a Semmelweis Egyetem Szenátusi termében (Bp. Üllői út 26. I. emelet).
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