Thesis supervisor: Györgyi Ferenc
co-supervisor: Dénes Dudits
Location of studies (in Hungarian): HAS Biological Research Centre, Szeged, Institute of Plant Biology Abbreviation of location of studies: SZBK
Description of the research topic:
Oligonucleotide Targeted Nucleotide Exchange (OTNE) is a technique for induction of gene specific mutation in plant genomes by synthetic single stranded DNA oligonucleotides (SDOs). For improving the efficiency of this methodology, we will synthesize SDO molecules with various length and chemical structures and test different uptake protocols such as bombardment of cells, or use of protoplasts. Using cultured maize cells, the mutation frequency will be monitored by green fluorescent protein marker (GFP) or herbicide resistance.
In parallel with modification of SDOs, the efficiency of TNE will be studied by the combination of SDOs with CRIPSR/Cas system. In this case double strand breaks (DSBs) induced by Cas9 will trigger the DNA repair pathways homology-directed repair (HDR). For this path first we will optimize the CRISPR/Cas system for cultured plant cells.
Required language skills: Hungarian Recommended language skills (in Hungarian): English Number of students who can be accepted: 1
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