Thesis supervisor: Melinda Pirity
Location of studies (in Hungarian): Embryonic and Induced Pluripotent Stem Cell Group, Institute of Genetics, Biological Researche Centre Abbreviation of location of studies: SZBK
Description of the research topic:
Ring1 and YY1-Binding Protein (RYBP) is a transcriptional regulatory protein and a central, essential member of mammalian Polycomb Repressive Complexes (PRC1). PRC1 complexes are involved in the formation of the long-term, so-called epigenetic memory of cells. As a result of their operation, during embryonic development, the expression of certain genes ceases, while those necessary for the given developmental stage are activated. This is how first differentiated cells (nerve, muscle, heart, etc.) are formed from the zygote, and hereafter increasingly complex tissues and finally organs arise.
Our group has shown that the function of RYBP is essential for embryo implantation, and for the development of the nervous system. We are looking for a motivated PhD student, who investigates the role of RYBP in neural and cardiac development in an in vitro mouse stem cell system. As a result of the work, it is expected that our knowledge of embryonic development, the formation of neural progenitors, oligodendrocytes, astrocytes and cardiac muscle cells will increase.
The specific goals of the project are: mapping of interactions between Rybp and the target neural genes.
Methods: stem cell cultivation, in vitro differentiation, Western-blot analysis, Fluorescent immunocytochemistry, RNA isolation, Reverse transcription, Real-time PCR.
Required language skills: English Number of students who can be accepted: 2
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