Investigation of the regulatory role of cytoskeletal Septin7 protein and its interaction partners in skeletal muscle under physiological conditions and in a muscular dyst
Thesis supervisor: Mónika Gönczi
Location of studies (in Hungarian): Department of Physiology, Faculty of Medicine, University of Debrecen Abbreviation of location of studies: DEÉI
Description of the research topic:
In skeletal muscle the cytoskeleton plays a prominent role in regulating the localization and interconnection of receptors, ion channels and regulatory molecules within the sarcomere. Our aim is to map the function of septins, considered as the fourth element of the cytoskeletal system, in the development of skeletal muscle, in its differentiation, and in muscle regeneration. During our previous experiments, we described the expression and localization of different septin proteins, as well as the morphological and functional changes that occur when the expression of the unique Septin7 is reduced in the C2C12 muscle-derived cell line and mouse skeletal muscle.
In our further experiments, our goal is to describe the proteins functionally related to Septin7, focusing on the molecules involved in muscle development, regeneration and calcium homeostasis of skeletal muscle (e.g. Orai1 and STIM1, which are important in SOCE). We also want to detemine whether the observations and protein-protein interactions described above are modified in the mdx muscular dystrophy mouse model, and if so, at what point and what mechanisms are modified. During the tests, the samples are examined at the RNA and protein level, and the location of the proteins within the cell/muscle fiber is analyzed by confocal microscopic analysis following immunostaining. We perform immunoprecipitation and mass spectroscopic analysis to map protein-protein interactions. To investigate muscle development, we would like to generate primary cultures from satellite cells isolated from the skeletal muscles of newborn mice and perform their appropriate analysis.
Required language skills: English intermediate level Number of students who can be accepted: 1
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